?HeLa
HeLa發(fā)音
英： 美：
HeLa中文意思翻譯
Henrietta Lacks strain of cancer cells 海拉癌細(xì)胞株
HeLa常見(jiàn)例句
1 、TCS1 and TCS2 have no obvious effect on HepA-H cells, but have a significant inhibitory effect on HeLa cells, indicating that TCS2 is superior to TCS1 in anti-tumor activity by the way of inducing apoptosis.───HepA-H細(xì)胞對(duì)天花粉蛋白不敏感,而HeLa細(xì)胞對(duì)TCS1和TCS2敏感,其中TCS2抑癌活性明顯強(qiáng)于TCS1,細(xì)胞生長(zhǎng)受抑制作用顯著,作用機(jī)制與誘導(dǎo)細(xì)胞凋亡相關(guān)。
2 、Cytotoxic effect and radiation enhancement of docetaxel in uterine cervical carcinoma cell line Hela───多西紫杉醇對(duì)宮頸癌細(xì)胞系Hela細(xì)胞毒性和放射增敏作用的研究
3 、Hela first, then Manya threw down muff and satchel and began excitedly choosing their own apples.───先是海拉，接著是瑪尼婭，都脫掉手套，放下書包，高興地挑選起自己要的蘋果來(lái)。
4 、The TEM and SEM observation of apoptotic Hela cells infected by HSV-1───HSV-1致Hela細(xì)胞凋亡的掃描電鏡及透射電鏡觀察
5 、incubation were fibroblasts,HeLa, BEL-7402,Detroit-6,SGC-7901,MGC 80-3 and Os-732 respectively according to the amount of ~3H-HPD in cells.───但在以后的24h 內(nèi),維持在類似的水平上。 7株細(xì)胞溫育30min 攝入~3H-HPD 量,按多少順序依次為成纖維細(xì)胞、HeLa、BEL-7402、Detroit-6、SGC-7901、MGC 80-3 及 Os-732;
6 、Effects of ESAC on in vitro growth of 3 cell lines (including human hepatoma BEL 7402,Human cervix uteri tumor-derived HeLa and human umbilical vein endothelial cell line HUVEC) were determined by MTT method.───利用MTT法檢測(cè)ESAC對(duì) 3種細(xì)胞系 (人肝癌系BEL74 0 2、人宮頸上皮癌細(xì)胞系HeLa和人臍靜脈血管內(nèi)皮細(xì)胞系HUVEC)體外生長(zhǎng)的影響。
7 、pE128 showed Rev dependent expression in Hela cell.───pE12 8在Hela細(xì)胞中顯示rev依賴性表達(dá) ,prev不存在時(shí) ,幾乎沒(méi)有EGFP表達(dá) ;
8 、STUDY ON CENTROMERIC DOTS OF HELA CELLS───HeLa 細(xì)胞染色體著絲粒點(diǎn)變異研究
9 、THE COMPARISON OF SENSITIVITY OF BGM CELLS, HELA CELLS, HEL CELLS AND A 549 CELLS TO HERPES SIMPLEX VIRUS 1───BGM細(xì)胞,Hela細(xì)胞,HEL細(xì)胞與A549細(xì)胞對(duì)HSV-1敏感性的比較
10 、Radiosensitization effect of N-4-hydroxyphenyl retinode on cervical carcinoma cell line HeLa───4-羥苯基維胺脂對(duì)宮頸癌HeLa細(xì)胞系的放射增敏作用
11 、Hela cells were also injected to normal BALB/c and Kunming mice.───同時(shí)用Hela細(xì)胞作為**腫瘤細(xì)胞對(duì)照，并接種于免疫功能正常的BALB/c小鼠和昆明種小鼠。
12 、Experimental study of the low dose cisplatin enhancing the apoptosis of HeLa cell induced by radiotherapy───小劑量順鉑增強(qiáng)放療誘導(dǎo)HeLa細(xì)胞凋亡的實(shí)驗(yàn)研究
13 、The inhibitory concentrations of iso-CPT to ECV304 and colo-16 cell were lower than those to tumor cells Tca8113 and Hela.───喜樹異堿抑制角質(zhì)形成細(xì)胞colo-16和血管內(nèi)皮細(xì)胞ECV-304增殖的濃度小于抑制舌鱗癌Tca8113細(xì)胞和宮頸癌Hela細(xì)胞的濃度。
14 、Gpd-Tp92 fusion gene constructed in pcDNA3.1 (+) vector could express a fusion protein in Hela cells.───pcDNA3.1(+)/Gpd-Tp92在HeLa細(xì)胞中能有效表達(dá)。
15 、HEAT-KILL KINETICS IN CELL CYCLE OF HeLa CELLS───Hela細(xì)胞的熱療動(dòng)力學(xué)研究
16 、Hela said the remainder of our mortal lives won't last long.─── 海拉說(shuō)過(guò) 我們的生命不會(huì)持續(xù)太久
17 、Role of mitochondria in Tubeimoside I-mediated poptosis in human cervical carcinoma HeLa cell line───土貝母苷甲在人宮頸癌HeLa細(xì)胞內(nèi)對(duì)線粒體的影響
18 、Molecular Cloning and Sequencing of P16 ink4 cDNA from Hela Cell───P16~(ink4)cDNA的克隆及序列分析
19 、Influence of Cardinal on Calcium Beaconage of Hela Cells───半邊蓮對(duì)Hela細(xì)胞鈣信號(hào)系統(tǒng)的影響
20 、RT-PCR showed that E1A gene had a stable transfection in Hela cells and obviously down-regulated Her-2 expression.───RT-PCR結(jié)果顯示:E1A基因在Hela細(xì)胞中能穩(wěn)定表達(dá),E1A基因的轉(zhuǎn)染明顯降低了Her-2基因在Hela細(xì)胞中的表達(dá)水平;
21 、The results of RT-PCT show that mRNA of p53 increase, while mRNA of p21 decrease then increase , and the results of Flow Cytometer show that HeLa cells accumulated in S-phase in one cell cycle.───RT-PCR結(jié)果顯示P53mRNA表達(dá)升高，而P21mRNA 表達(dá)為先降低后升高，并且流式細(xì)胞實(shí)驗(yàn)結(jié)果顯示HeLa細(xì)胞在一個(gè)細(xì)胞周期時(shí)間內(nèi)細(xì)胞主要積累在S期。
22 、MTT assay was used to examine the growth rates rates inhibition of AIDI on human hepatoma(BEL7402),human cervical carcinoma(HeLa),human melanoma(A375-S2),human gastric carcinoma(SGC-7901) and mice fibroma sarcomatosum(L_(929)).───體外實(shí)驗(yàn):常規(guī)傳代培養(yǎng)人肝癌BEL7402、人宮頸癌HeLa細(xì)胞、人黑色素瘤A375 S2細(xì)胞、人胃癌SGC 7901和小鼠纖維肉瘤L929細(xì)胞,用MTT法檢測(cè)活細(xì)胞數(shù)和生長(zhǎng)抑制率。
23 、uterine cervix eaneer cell HeLa───宮頸癌細(xì)胞
24 、Cervical Neoplasms Hela cell line───宮頸癌Hela細(xì)胞
25 、Determined by colonal formation, the inhibition rate of EWE (4mg/ml) against 7721, Hela, MKN-45 cells were 59.8%, 66.4%, 70.5%.───EWE4mg/ml對(duì)三種細(xì)胞集落抑制率分別為59.8%、66.4%、70.5%。
26 、With human tumor stem cell assay, CATC inhibited the stem cell growth of Hela and QGY7703 cell lines.───CATC對(duì)小牛主動(dòng)脈血管內(nèi)皮細(xì)胞有明顯抑制作用。
27 、Keywords soybean trypsin inhibitor;the human hela cells;cell multiplication;───大豆胰蛋白酶抑制劑;人宮頸癌細(xì)胞;細(xì)胞增殖;
28 、Keywords S.acus Linnaeus exact;Hela;anti-cancer effect;MTT;───尖海龍?zhí)崛∥?瘤細(xì)胞株;倒置顯微鏡;抗癌作用;
29 、Detection of the Virus RNA from HeLa Cells Infected with? Coxsackievirus B_3 by in Situ RT-PCR───原位RT-PCR法檢測(cè)柯薩奇B_3病毒感染HeLa細(xì)胞中的病毒RNA
30 、Hela knew as soon as we tried to escape, our life force would start fading.─── 海拉知道 一旦我們嘗試逃離 我們的生命力就會(huì)開始衰退
31 、OBSERVATIONS ON THE GROWTH DYNAMICS OF 4 STRAINS OF TOXOPLASMA GONDII IN HELA CELLS───四株弓形蟲在HeLa細(xì)胞內(nèi)生長(zhǎng)動(dòng)態(tài)的觀察
32 、Construction of Expressing Vector of Fusion Gene of EGFP and ALR and Its Expression in Hela Cells───EGFP和ALR融合基因表達(dá)載體構(gòu)建及其在HeLa細(xì)胞中的表達(dá)
33 、Mechanisms of apoptosis induced by PBA-D1 in Hela cells───土槿乙酸衍生物PBA-D1誘導(dǎo)Hela細(xì)胞凋亡及其機(jī)制
34 、Keywords Chlamys nobilis;Glycosaminoglycan;Hela cell;inhibited effect;───華貴櫛孔扇貝;糖胺聚糖;人宮頸癌細(xì)胞;抑制作用;
35 、Obtainment and sequencing of the cDNA derived from the non-structural P2C of CVB4 First,we inoculated CVB4 into Hela cells which had been cultivated and spreaded the bottom of culture bottles.───一、柯薩奇B4病毒非結(jié)構(gòu)蛋白P2C CDNA的獲取 首先采用細(xì)胞培養(yǎng)技術(shù)，當(dāng)人宮頸癌細(xì)胞（HeLa ）長(zhǎng)滿后,接種柯薩奇B4病毒，細(xì)胞病變達(dá)到80%時(shí)及時(shí)收獲病毒。
36 、With FuGENE6 transfection reagent, AIRE gene was stably expressed in HeLa cells that would be helpful for further study on AIRE gene.───在 Fu GENE6轉(zhuǎn)染試劑的介導(dǎo)下 ,AIRE基因在 He La細(xì)胞內(nèi)獲得穩(wěn)定表達(dá)
37 、Construction of expression vector of follicular inhibin and HBsAg fusion gene and expression in Hela cell───卵泡抑制素與**表面抗原融合基因表達(dá)質(zhì)粒的構(gòu)建及表達(dá)
38 、Effects of Smac gene over-expression on radiotherapeutic sensitivity of cervical cancer cell line HeLa───Smac基因過(guò)表達(dá)對(duì)宮頸癌HeLa細(xì)胞放療敏感性的影響
39 、Study on the Biological Properties of Solubilized HeLa Cell Receptors for Group B Coxsackieviruses───可溶性HeLa細(xì)胞柯薩基B組病毒受體性質(zhì)的研究
40 、but the inhibition rate was cut down to 28% in Hela and 13% in HepG2 cells lines.───但在Hela細(xì)胞中,抑制作用明顯減低至28%; 在HepG2細(xì)胞中抑制作用更弱,僅為13%。
41 、The telomerase activities of HeLa,Tca-8113,YTLMC-90 and Eca-109 cells in vivro were investigated by TRAP-ELIAS technique.───為了進(jìn)一步證實(shí)異鼠李素的抑癌抗癌作用，對(duì)其抑制腫瘤生長(zhǎng)增殖和誘導(dǎo)腫瘤細(xì)胞凋亡的分子機(jī)制進(jìn)行了實(shí)驗(yàn)研究。
42 、human cervical carcinoma Hela cell───人宮頸癌Hela細(xì)胞
43 、Low dose cisplatin can enhance the sensitivity of radiotherapy through change cell cycle of Hela cell lines.───小劑量順鉑可通過(guò)改變培養(yǎng)的人Hela細(xì)胞周期 ,誘導(dǎo)細(xì)胞G2 /M期延遲從而提高放療敏感性 ,可能與其內(nèi)在的 p5 3基因表達(dá)改變有關(guān)。
44 、Application of Microarray in Study on Apoptotic Mechanism of HeLa Cells Induced by HPV18 E6 siRNA and HPV Detection───基因芯片技術(shù)在HPV18 E6 siRNA誘導(dǎo)HeLa細(xì)胞凋亡機(jī)制研究及HPV檢測(cè)分型中的應(yīng)用
45 、Hormesis and adaptive response of survival in HeLa cells induced by low dose X-ray irradiation───低劑量X射線照射誘導(dǎo)HeLa細(xì)胞存活的
46 、KGF and KGFR are expressed in Hela cells.───Hela細(xì)胞中有KGF和KGFR的表達(dá);
47 、Keywords cell fusion;red blood cells;Hela cells;───關(guān)鍵詞細(xì)胞融合;雞紅細(xì)胞;聚乙二醇;
48 、Tubeimoside I-induced ultrastructural changes of human cervical carcinoma HeLa cell line and the protective effect of cyclosporine A───土貝母苷甲誘導(dǎo)的人宮頸癌HeLa細(xì)胞凋亡的超微結(jié)構(gòu)變化及環(huán)孢菌素A的保護(hù)作用
49 、Effect of anticancer polypeptide from Buthus Martensii Venom (APBMV) on Eca 109 cells and Hela cells───東亞鉗蝎毒抗癌多肽對(duì)Eca109 細(xì)胞和HeLa 細(xì)胞的毒性作用
50 、Effect of the truncated human apoptosis-inducing factor expression on apoptosis of HeLa cells───人截短型凋亡誘導(dǎo)因子的表達(dá)對(duì)HeLa細(xì)胞的促凋亡作用
51 、Keywords Uterine cervix carcinoma;Proliferating cell nuclear antigen;Small hairpin RNA;Hela cell;───關(guān)鍵詞子宮頸癌:增殖細(xì)胞核抗原;小發(fā)夾結(jié)構(gòu)RNA;子宮頸癌細(xì)胞;
52 、The objective was designed to investigate the function of VEGF-C in cervical cancer cell line Hela, proliferation, adhesion.───對(duì)腫瘤淋巴轉(zhuǎn)移的調(diào)控，是否也與其對(duì)腫瘤細(xì)胞自身增殖、黏附、侵襲等，轉(zhuǎn)移相關(guān)生物學(xué)行為的影響有關(guān)。
53 、Radiosensitivity and growth characteristics of filial generation from irradiated HeLa cells───HeLa細(xì)胞照射后存活后代生長(zhǎng)特性及放射敏感性的變化
54 、COMPARISON OF INTAKES OF ACM-LDL COMPLEX AND FREE ACM BY HELA CELLS OF UTERINE CERVICAL CARCINOMA───宮頸癌HeLa細(xì)胞攝取ACM-LDL及游離ACM的比較研究
55 、Stability of p21 WAF/CIP1 Regulated by Skp2 in HeLa Cells───HeLa細(xì)胞中Skp2的表達(dá)調(diào)控p21WAFCIP1穩(wěn)定性
56 、ELEMENTARY DETECTION OF CORRELATION?BETWEEN PKA, PKC AND CDC2 ACTIVITY?IN G2/M/G1 PROGRESSING OF HeLa CELLS───HeLa細(xì)胞G2/M/G1進(jìn)程中PKA、PKC與CDC2活性變化相關(guān)性之初探
57 、Ang-2 cDNA were subcloned into pcDNA3 vector and transfected into Hela cell lines by the lipofection method.───將Ang-2的cDNA克隆到真核表達(dá)載體pcDNA3上，應(yīng)用脂質(zhì)體轉(zhuǎn)染技術(shù)將該真核表達(dá)載體穩(wěn)定轉(zhuǎn)染人Hela細(xì)胞。
58 、We found that the anti-HCMV-pp65-3 antibody from previously immunized animals cross react to unknown proteins from Hela extract.───在動(dòng)物實(shí)驗(yàn)中，施打pp65-3 蛋白質(zhì)，得到抗pp65-3 抗體，會(huì)與Hela 抗原中的未知蛋白質(zhì)產(chǎn)生交叉反應(yīng)。
59 、The Studies on Dynamic Relationship between HeLa Nucleolar rDNA Transcription, Pre-rRNA Processing and the Ultrastructure───HeLa細(xì)胞核仁rRNA基因轉(zhuǎn)錄和pre-rRNA剪切與超微結(jié)構(gòu)動(dòng)態(tài)關(guān)系研究
60 、Determined MTT staining, the IC50 values of EWE against 7721, Hela, MKN-45 cells were 1.43, 1.67, 0.97mg/ml.───EWE對(duì)三種細(xì)胞降解MTTIC_(50)值分別為 1.43、1.67、0.97mg/ml;
61 、REVERSIBILITY OF THE EARLY G_1 BLOCKING OF THE ASYNCHRONOUS AND M-SYNCHRONIZED HeLa CELLS TREATED BY N-BUTYRATE───丁酸鈉對(duì)M期同步及非同步的HeLa細(xì)胞早G_1期阻斷的可逆性
62 、Suppressive effect on HeLa cells proliferation by phenothiazine derivatives alone and combining with ionizing radiation───吩噻嗪衍生物協(xié)同放射對(duì)HeLa細(xì)胞增殖的抑制效應(yīng)比較研究
63 、Curcumin can induce apoptosis of HeLa cells to kill the tumor cells by suppressing the expression of HVP E6 to restore the function of P53.───姜黃素通過(guò)抑制HPV E 6的表達(dá),恢復(fù)P 53的功能,引起細(xì)胞凋亡,起到殺傷腫瘤的作用。
64 、The Killing Effect of S. Acus Linnaeus Exact on Hela───倒置顯微鏡觀察尖海龍?zhí)崛∥飳?duì)Hela細(xì)胞的殺傷作用
65 、Optimal Catalytic Conditions of HeLa Cell Telomerase and Reconstitution of Human Telomerase Activity───HeLa細(xì)胞端粒酶最適反應(yīng)條件及活性重建
66 、To observe direct inactivate effect of PAP to CBV3 by microdose cytopathogenic effect inhibition assay in Hela cell cultures.───在Hela細(xì)胞上采用微量細(xì)胞病變抑制法觀察PAP直接滅活CBV3的作用。
67 、Effect of Bak on regulation of cell cycle and apoptosis in Hela cells───Bak基因?qū)ela細(xì)胞的細(xì)胞周期和細(xì)胞凋亡的調(diào)控作用(英文)
68 、Either the cell growth inhibitory rate or the colony inhibitory rate of the HeLa cells was 100%(P0.05).───12 Gy的輻射劑量能夠完全抑制腫瘤細(xì)胞的增生(細(xì)胞生長(zhǎng)抑制率及克隆形成抑制率均為100%,P0.05)。
69 、Hela human cervical carcinoma cell───人宮頸癌Hela細(xì)胞株
70 、Utilized radioactive immunosorbent assay, cAMP accumulation and protein kinase A(PKA) activity in HeLa cells and the effects of AFP were detected on the concentration and activity in the cells pretreated with pertussis toxin (PTX).───再用放射免疫結(jié)合法分析在百日咳毒素 (pertussistoxin ,PTX)預(yù)處理前后AFP對(duì)細(xì)胞內(nèi)環(huán)腺苷酸 (cAMP)濃度及細(xì)胞內(nèi)蛋白激酶A(proteinkinaseA ,PKA)活性變化的影響 .
71 、Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells.───將上述基因克隆入綠色熒光蛋白(GFP)表達(dá)載體pIRES2-EGFP中,轉(zhuǎn)染人HeLa細(xì)胞,在熒光顯微鏡和電鏡下觀察轉(zhuǎn)染細(xì)胞的形態(tài)和結(jié)構(gòu)。
72 、Establishment of a two-dimensional electrophoresis technique for proteome of hela cells───hela細(xì)胞蛋白質(zhì)組雙向電泳技術(shù)的建立
73 、Specific Suppression of HPV18 E6 Oncoprotein by RNA Interference in Hela Cells───RNA干擾技術(shù)特異抑制HPV18E6基因表達(dá)的研究
74 、The KGF and KGFR genes were expressed in the Hela cells. The KGF and KGFR proteins were expressed in the Hela cells.───從基因水平和蛋白水平證實(shí)KGF及KGFR在Hela細(xì)胞中有表達(dá)。
75 、Different phenotypes of P68 between tumouric cell li nes (HeLa, TC 3H 10) and non-tumouric cell lines (NIH3T3 and NC 3H 10 ) were observed, and we reasoned that overexpression of P68 might profoundly a ffect cellular growth properties.───以前的工作表明P68核蛋白在同一細(xì)胞系不同生長(zhǎng)階段的表型不同。 它在腫瘤細(xì)胞 (HeLa和TC3H10 )與非腫瘤細(xì)胞 (NIH3T3和NC3H10 )之間表型亦有明顯差異。
76 、Using PCR, we also obtained the cDNA sequence of PRPF8 C-terminal and using co-immunoprecipitation experiments, we confirmed the interaction between PAI-2 and PRPF8 in HeLa cells.───另外我們也獲得了PRPF8羧基端的部分序列，進(jìn)一步用免疫共沉淀的實(shí)驗(yàn)證實(shí)，PAI-2與PRPF8在HeLa細(xì)胞內(nèi)確實(shí)存在相互作用。
77 、The s-lap/GFP fusion protein can express in Hela cells and locate in entire cells, but express highly in the nucleus.───s- lap/ GFP融合蛋白可在人 Hela細(xì)胞中表達(dá) ,并主要**于細(xì)胞核。
78 、Cloning of mouse Nanog gene and its effect on human Hela Cells───小鼠Nanog基因的克隆及對(duì)人宮頸癌上皮細(xì)胞的作用
79 、That electroporation can induce apoptosis is investigated in HeLa cells, the effects of electroporation to apoptosis of HeLa cells is studied with Giemsa,Acridine orange,Hoechst33342 and propidium iodide staining.───作者報(bào)道了電穿孔可以誘發(fā)HeLa細(xì)胞凋亡 ,采用Giemsa染料、吖啶橙染色及Hoechst33342 ,PI雙染的熒光染色 ,研究了電穿孔對(duì)HeLa細(xì)胞凋亡的影響 .
80 、Keywords P;Targeted radiotherapy;Radiobiological mechanism;HeLa cell lines;Apoptosis;Cell proliferation;Cell cycle;───關(guān)鍵詞32P;靶向治療;放射生物學(xué);細(xì)胞系;凋亡;細(xì)胞增殖;細(xì)胞周期;
81 、RT-PCR demonstrated that E1A gene had a stable transfection in Hela cells and obviously down-regulated HER-2/neu expression.───RT-PCR結(jié)果顯示:E1A基因在宮頸癌細(xì)胞中能穩(wěn)定表達(dá)并且顯著降低HER-2/neu在宮頸癌細(xì)胞中的表達(dá)水平。
82 、Human cervical carcinoma cell line HeLa───人宮頸癌細(xì)胞株HeLa
hela細(xì)胞高表達(dá)的基因有哪些
HeLa細(xì)胞通過(guò)關(guān)閉某些信號(hào)通路(包括PI3K和MKK4/MAPK信號(hào)通路),激活某些信號(hào)通路(包括cAMP、Ca~(2+)—CaM、PKC、Ras和P38/MAPK信號(hào)通路),激活或抑制不同的信號(hào)分子,使細(xì)胞產(chǎn)生具有針對(duì)性的細(xì)胞效應(yīng),包括誘導(dǎo)表達(dá)多種特異性蛋白(酶、細(xì)胞骨架蛋白、細(xì)胞因子等)、改變代謝途徑、減弱某些代謝、加強(qiáng)另一些代謝以及提高胞內(nèi)運(yùn)輸能力等,最終使自己可以適應(yīng)痢疾桿菌的侵襲而生存下去。 為了鑒定在病原菌與寄主細(xì)胞相互作用中新的未知的EST序列,我們采用代表約4000個(gè)新基因的cDNA微陣列研究了痢疾桿菌入侵前和入侵后3h的人上皮細(xì)胞差異表達(dá)的新基因,結(jié)果共鑒定到≥3倍或≤0.33的差異表達(dá)的代表新基因的EST序列76條,然后這些EST序列采用Siclone軟件分析,進(jìn)行電子延伸,在BLAST比較分析之后,其中25個(gè)延伸序列鑒定為與重要的腸道功能相關(guān)的已知基因,包括痢疾桿菌-誘導(dǎo)的凋亡(TPPⅡ)、細(xì)胞骨架(如actin、talin、cofilin和gelsolin)、離子轉(zhuǎn)運(yùn)(calpain-5、Nedd4和Calcium-transporting ATPaseⅠ)、離子通道功能(如ABCA8、TPD52等)、粘膜屏障防御(CLECSF2)、異型生物質(zhì)代謝以及上皮細(xì)胞的保護(hù)機(jī)制(vof-16)。更為重要性的是,通過(guò)RT-PCR克隆并測(cè)序,經(jīng)BLAST搜索,鑒定到3個(gè)顯著差異的新的EST序列,并用熒光實(shí)時(shí)定量RT-PCR實(shí)驗(yàn)證實(shí)這三個(gè)新序列的確在痢疾桿菌侵襲期間高表達(dá)。這三個(gè)基因是新的人基因或假基因,其相關(guān)信息已提交GeneBank,登錄號(hào)為nos.AY776160,AY776161和AY776162。我們認(rèn)為它們?cè)诩?xì)胞對(duì)痢疾桿菌2457T侵襲反應(yīng)中起重要的作用。這些結(jié)果促進(jìn)了對(duì)痢疾桿菌分子致病機(jī)理的認(rèn)識(shí),也為形成預(yù)防和治療痢疾的策略提供了理論基礎(chǔ)。
hela細(xì)胞轉(zhuǎn)染后幾小時(shí)死亡是什么原因
hela細(xì)胞轉(zhuǎn)染后幾小時(shí)死亡是什么原因
可能有多種原因： 目標(biāo)蛋白對(duì)細(xì)胞有毒性，導(dǎo)致細(xì)胞死亡； 轉(zhuǎn)染試劑以及DNA用量信息需要優(yōu)化，否則對(duì)細(xì)胞具有傷害； 細(xì)胞貼壁轉(zhuǎn)染之后沒(méi)有正常換液。 建議： 考慮對(duì)目標(biāo)蛋白進(jìn)行截短構(gòu)建、嘗試其他細(xì)胞系統(tǒng)； 摸索轉(zhuǎn)染試劑以及DNA用量信息，如果轉(zhuǎn)染試劑毒性太大，可以考慮嘗試義翹轉(zhuǎn)染試劑sinofection； 對(duì)轉(zhuǎn)染后的細(xì)胞進(jìn)行換液處理，如果細(xì)胞狀態(tài)感覺(jué)不夠理想，可以考慮添加一些血清來(lái)幫助細(xì)胞恢復(fù)健康。 以上所有分析、建議的前提是，細(xì)胞培養(yǎng)、無(wú)菌操作等等都沒(méi)有問(wèn)題。祝順利，加油~